Genetics Algorithm MCQ
In this article, we will discuss the most commonly asked multiple-choice questions related to the Genetics Algorithm.
The main purpose of writing this article is to target competitive exams and interviews. Here, we will try to cover all the frequently asked Genetics Algorithm questions with the correct choice of answer among various options.
1) What will happen when a chain-termination mutation is found in the S gene?
Answer: a) Cell lysis gets blocked
Description: Cell lysis gets blocked whenever a chain termination mutation is found in the S gene. Mainly, high levels of packaging protein are found in the S gene, which is then carried out artificially.
2) The endonuclease cuts the DNA from __________.
Answer: b) within the polynucleotide chain, and not at the ends
Description: The endonuclease cuts the DNA from within the polynucleotide chain and not at the ends, whereas exonuclease cuts the sequence at the ends.
3) What protects the modified DNA after undergoing replication?
Answer: b) Semi-conservative mode of replication
Description: Due to the semi-conservative mode of replication, one of the strands of DNA remains methylated after undergoing replication. A single methylated strand of DNA is sufficient enough to protect the DNA against cleavage by restricting endonuclease.
4) What is the name of the phenomenon in which the specificity of the enzyme gets affected by the concentration of the used buffer?
Answer: c) star activity
Description: The star activity refers to a phenomenon in which the specificity of an enzyme gets lost whenever a concentration buffer is varied.
5) The recognition sequence for BamHI is 5'G|GATCC 3', where '|' represents the cutting site. What can be concluded by the ends?
Answer: b) The single-stranded end is found to be 5' in nature
The other strand is nothing but complementary to it and can be written in the following way:
5' G|GATCC 3'
3' CCTAG|G 5'
After cleavage, the sequences are represented as:
5' G 3' 5' GATCC 3'
3' CCTAG 5' 3' G 5'
Thus, we can see that the ends generated are single-stranded at 5' end.
6) Which of the following statement is true in terms of partial digestion?
Answer: a) Partial digestion can be defined as a condition which does not recognize any of the site present in the DNA sequence.
Description: Since partial digestion cannot recognize all of the sites, it can never create a similar number of fragments as complete digestion. It is beneficial in genomic library representation because it can represent nearly each and every segment.
7) How many types of polymerases are there in basic classification?
Answer: d) 5
There are 5 types of polymerases in basic classification, which are as follows:
8) What is meant by polymerase?
Answer: d) An enzyme that can be utilized to synthesize a new DNA or RNA strand based on the pre-existing strand or without it
Description: The synthesis of a new strand of DNA or RNA can be done either based on the pre-existing strand or without it. The strands which do not necessitate a pre-existing strand refer to a template-free or template independent.
9) Which of the following activity can't be seen in the case of DNA polymerase?
Answer: d) 3'-5' DNA synthesis
Description: DNA polymerase encompasses an ability to synthesize DNA strands only in the direction of 5'-3' and not in 3'-5' direction. However, it can possess exonuclease activity in both directions, which means that it can remove bases in either direction but can synthesize only in 5'-3' direction.
10) Which of the following represent the correct mode of actions of exonuclease III?
Answer: b) Exonuclease III acts on DNA's double strands in 3'-5' direction
Description: An enzyme that possesses exonuclease activity on DNA's double strands in 3'-5' direction and not on single-stranded DNA molecules.
11) How to protect one end from Exonuclease III's action to prevent the molecule from getting shortened from both ends?
Answer: a) By utilizing Phosphonothioate nucleotide analogue
Description: Phosphorothioate nucleotide analogues replace the nucleotides at the ends. By restricting the enzyme from performing any action, it makes sure that the molecule does not get shortened at the end where the replacement is done.
12) The primary function of methylase is to __________.
Answer: a) Add the methyl groups to the DNA
Description: Methylase is used to add the methyl groups to the DNA. It can be done by placing the methyl groups taken from S-adenosyl methionine.
13) What is meant by litigation?
Answer: c) Alignment of either of the double or single-stranded DNA molecules and the formation of phosphodiester bonds. The bond can be between either one or both the strands
Description: Ligation refers to the formation of bonds amid the ends of two DNA strands. The strands can either be single or double. Since the nature of the bond is found to be phosphodiester, thus the bond is mainly formed amongst sugar and phosphate. The phosphodiester bond can either be formed between one strand or both strands.
14) In which of the following cases, litigation reaction is found to be more efficient?
Answer: a) Sticky end litigation
Description: In contrast to blunt and litigation, sticky end litigation is found to be more efficient because it is carried out due to complementary base pairing.
15) If recircularization is observed while carrying out a litigation reaction, what does it infers to?
Answer: b) Intramolecular
Description: Recircularization refers to such phenomena in which the ends of the same molecules are joined all together. It happens in the case of an intramolecular ligation reaction.
16) Increase in reaction component's concentration leads to __________.
Answer: b) Litigation only in intermolecular without affecting intramolecular
Description: As the concentration of reaction components increases, there are increased chances of ligation in intermolecular reaction because of the frequency of collision of two different molecules. The intramolecular reaction is unaffected because the probability of meeting the ends of a molecule remains the same.
17) What is the name of the enzyme that is commonly used for carrying out litigation reactions?
Answer: a) Ligase
Description: The ligase enzyme helps in carrying out the ligation reaction, which can be either obtained from E. coli or from cells infected by the virus.
18) Which of the following enzyme can be used to carry blunt-ended litigations?
Answer: a) T4 DNA Ligase
Description: T4 DNA ligase helps carry out the blunt-ended ligations as E. coli DNA ligase cannot carry out blunt-ended ligation.
19) __________ enzyme helps in carrying out elements of mobile genetics from one portion of DNA to another.
Answer: a) Transposase
Description: Transposase transfers a mobile genetic element from one portion to another, which helps in inserting the origin of replication or anti-biotic resistance genes.
20) Many bacterial species have a natural ability to take up the exogenous DNA material. Which of the statement is not correct in regard to it?
Answer: a) It is not limited to particular growth phases
Description: This ability is termed competence, and it is limited to particular growth phases. It results in developing new biochemical abilities under special conditions such as nutrient deprivation. If the bacterial cells are naturally competent, they may take the foreign DNA by simply incubating them with foreign DNA.
21) Viral infection can be used to take up the DNA by cells.
Answer: a) True
Description: Viral infection can be used to take up the DNA by the cells due to the viral coat, and the nucleic acid contained within has no role to play.
22) As isolation of genomic DNA works similar to obtaining plasmid from E. coli, which of the following is not considered in it?
Answer: c) Dissolving plasmid in water
To obtain plasmid DNA from E. coli, some basic steps are involved.
23) In how many ways can we obtain the plasmid DNA from the bacteria?
Answer: b) 2
Description: Mainly, there are two methods called the alkaline lysis method and boiling lysis method with which the plasmid DNA can be obtained from the bacteria. Both of the methods follow different approaches.
24) Cell lysis is carried out by __________.
Answer: c) Lysozyme and detergents
Description: Cell lysis can be performed by adding lysozyme and detergents. The cell wall is formed by cross-linking N-acetyl glucosamine and N-acetyl muramic acid. The agents that are added to break the cross-links are present between the molecules of the cell wall.
25) __________ process is carried out to separate chromosomal or genomic DNA.
Answer: d) Centrifugation
Description: In general, chromosomal or genomic DNA is found to be heavier and large in size in contrast to plasmid DNA. Thus, centrifugation at high speed is performed to settling down the genomic DNA so that they can be easily separated.
26) Which of the following treatment can be used to remove the proteins?
Answer: b) Phenol and chloroform treatment
Description: The combination of phenol and chloroform helps to remove the proteins as chloroform cannot give sufficient results if used alone. The addition of phenol helps to destruct the proteins, followed by assisting the chloroform to dissolute under acidic conditions.
27) The nucleic acid remaining in the solution can be precipitated by the addition of sodium or ammonium acetate and ethanol.
Answer: a) True
Description: The nucleic acid is present in the solution and is precipitated by the addition of sodium or ammonium acetate and ethanol. It is because; nucleic acid is polar in nature and thus easily dissolves in water. Hence, to avoid this, sodium acetate and ethanol are added. Sodium acetate is shielding the charge present on the sugar-phosphate backbone, and further bonds are easily formed between ethanol and phosphate. It leads to separating out of nucleic acids.
28) Gel electrophoresis separates nucleic acid molecules based on __________.
Answer: b) size of molecules
Description: Based on the molecule's size, gel electrophoresis separates nucleic acid molecules. Due to the force applied by the electric field, the nucleic acid molecules have to pass through the gel.
29) If the DNA is equally distributed, which of the following is going to pass through the gel?
Answer: c) Supercoiled
Description: The supercoiled form of DNA travels the fastest because the movement through the gel is based on the molecule's size. The smaller the molecule, the less retarding force would be experienced during the movement. Hence, supercoiled having the smallest size will move the fastest.
30) In electrophoresis, which of the following method is used to recovery the DNA from gel?
Answer: d) Electro-elution
Description: Electro-elution is used to remove the DNA from the gel. Different electro-elution methods are used, such as dialysis tubes or membrane is used.
31) Which of the following statement is correct for the DNA recovery method, which is also called freeze-squeeze?
Answer: c) Once it is frozen, centrifugation is then performed with the help of a glass wool plug.
Description: The DNA recovery method is useful for procuring DNA from the gel. The slice, which is full of DNA, is broken down so as to freeze it in liquid nitrogen. Freezing in liquid nitrogen helps to disrupt the structure. Once it is frozen, centrifugation is then performed with the help of a glass wool plug, and the gel is retained. The liquid containing dissolved DNA is then passed through it.
32) Which of the gel portion results in the greatest separation?
Answer: b) Lower portion where the anode is present
Description: The greatest separation is obtained in the lower portion of the gel where the anode is present. Thus, the smaller molecules encompass the greatest separation as they can travel farther in the gel.
33) What if the molecules that are to be separated are found to be larger in size than that of the conventional electrophoresis, which of the following gel is preferred?
Answer: a) Pulse field gels
Description: Generally, when the larger size molecules or chromosomes are separated, pulse-field gels are used, called pulse-field gel electrophoresis (PFGE).
34) Which is of the following is true in terms of primers?
Answer: b) Primers are 20-30 nucleotides long
Description: In general, primers are found to be 20-30 nucleotides long. Short primers can easily match the template in comparison to long primers. However, when the template is eukaryotic DNA, then long primers are preferred.
35) _________ is the mathematical equation of melting temperature.
Answer: a) 4(G+C) + 2(A+T)
Description: The melting temperature refers to that temperature at which the primers associate with the template. The number of different nucleotides present decides the melting temperature.
36) Which of the following is incorrect with respect to sickle cell anemia, a genetic disorder?
Answer: c) The mutation takes place in the alpha globulin gene.
Description: Sickle cell anemia refers to a process of mutation that takes place in the beta globulin gene. It first destroys the restriction site, after which PCR analyzes it. In contrast to conventional approaches, PCR is found to be very fast as it needs just one day to complete the whole process.
37) Which of the following tissues enables the usage of permeabilized tissue?
Answer: a) In-situ PCR
Description: In-situ PCR allows the use of permeabilized tissue, such as thin sections on a microscopic slide. The PCR product is detected by hybridization, and it allows locating the nucleic acid in the target tissue.
38) On a general basis, which of the following characteristics are not found in the plasmid?
Answer: d) Beta galactose genes
Description: The plasmid is composed of characteristics such as multiple cloning sites, the origin of replication, antibiotic-resistant genes, and beta-galactosidase genes. In order to perform replicate, an origin of replication is used.
39) Alkaline Phosphatase is used at times to treat the vector. Which of the following is an incorrect statement?
Description: Alkaline phosphatase treats with the vector at the time of removing the phosphatase group from the 5' end. This group is required for the ligation reaction to take place, such that at each end, two phosphates are required. But as soon as the phosphate groups are removed from the vector, its relegation becomes impossible. And since the insert still has a phosphate group, one of its strands will form the bond, enabling a ligation reaction to take place.
40) What if we cut the insert DNA with two distinct restriction enzymes at the ends?
Answer: c) The insert can ligate in only one alignment
Description: If the DNA is cut with two different enzymes at the ends, then it may lead to ligate the fragment in only one orientation because each end would have a unique sequence to ligate.
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